In-Vitro Anti-Oxidant, Anti-Amylase, Anti-Arthritic and Cytotoxic Activity of Important Commonly Used Green Leafy Vegetables

Over the past two decades, there has been an increased recognition of the importance of wild or locally cultivated food plants as sources of micronutrients and plant secondary metabolites. Green leafy vegetables (GLVS) have proved over time that they provide more protective energy to human body than any other vegetable.The present study concern about Anti-Oxidant, Anti-Amylase, Anti-Arthritic and Cytotoxic properties of Commonly Used Green Leafy Vegetables i.e. Amaranthus viridis (AV), Amaranthus gangeticus (AG), Anethum sowa (AS). The phytochemical analysis of aqueous extract of three GLVS has indicated the presence of steroid, flavonoid, and saponins types of compounds.EC50 values of DPPH free radical and Nitric Oxide scavenging activity of AV, AG, AS and gallic acid standard were found to be 104.64 μg/ml, 110.34 μg/ml, 90.24 μg/ml, 88.42 μg/ml and 105.68 μg/ml, 109.46 μg/ml, 91.28 μg/ml, 87.58 μg/ml respectively. The AV, AG, AS exhibited appreciable α-amylase inhibitory activity with an IC50 values 32.24μg/ml, 47.86μg/ml, and 72.34μg/ml respectively, when compared with acarbose (IC50 value 82.72μg/ml).The maximum membrane stabilization and protein denaturation inhibition of AV extract was found to be at 90.42% and 70.48% respectively. Among the three extract tested AS (LC50-62.44μg/ml) showed more and AV(LC50-64.16μg/ml) AG(LC50-98.67μg/ml) showed moderate cyto toxicity activity compared with standard vincristine sulfate(LC50 of 0.52 μg/ ml) assayed by brine shrimp lethality bioassay. The results demonstrate that GLVs studied contained highly pharmacological active moiety. More work should carry out to isolate and characterize the chemical constituents to ascertain its pharmacological properties.